Workflow from Scientific Research

A scheme depicting the fractionation of complete conditioned medium by ultracentrifugation and filtration. CM, conditioned medium; CCM, complete conditioned medium; 2S, 10S, and 100S Fractions, supernatant obtained after centrifugation at 2000g, 10,000g, and 100,000g, respectively; 100P Fraction, pellet obtained from 100,000g centrifugation; Filtered, flow-through after filtration with 0.02 m-membrane filter.

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