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TRIMCyp restriction assay to assess the cyclophilin A binding capabilities of SiR-tetrazine-labeled CA* pseudovirions. TZM-bl cells expressing TRIMCyp were infected with CA*/untagged G89V pseudoviruses, and the resulting luciferase signal was measured after 48 h. Firefly luciferase light units were normalized to p24 ELISA content, and the CA*/untagged G89V ratio was normalized to 100% G89V infectivity (CA*:G89V:p= 0.0008, CAWT:G89V:p= 0.01, CA*:CAWT:p= 0.32, CAWT:G89V:p= 0.003, unpairedttest, mean and SD shown for two different viral preparations).
#Bar Plot#Error Bars#TRIMCyp Restriction Assay#Cyclophilin A#SiR-tetrazine#Pseudovirions#TZM-bl Cells
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