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PF74 time-of-addition based measurements of the nuclear import kinetics for SiR-tetrazine-labeled WT and CA* pseudoviruses. Here, 2.5 M PF74 was added to cells at 0, 2, 4, 8, 10, and 24 hpi (T50for CA* = 6.27 hpi,T50for WT CA = 6.58 hpi, mean and SD shown for three different viral preps).T50values for CAWTand CA* were not statistically different (p= 0.7, unpairedttest).
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