Matched Xenium and Visium HD images of mixed FABP4 + / SPP1 + (h) macrophage accumulations within distal airspaces. In h, the sum log 2 expression of a list of genes marking alveolar macrophages is compared to a list marking SPP1 + macrophages. Genes that were strong markers for both alveolar and SPP1 + macrophages were not included in h. See Methods for the gene selection process and Supplementary Table 10 for a list of marker genes. For c e, g, h Xenium images, all listed genes are potentially visible in each example image if expressed, except SCGB3A2, which for clarity is not shown on the two figures that include airways, and AGER , which is only shown in d . Scale bars on the bottom left of each H&E = 20 m. g, h, sample VUILD49LA (cHP).
