Scheme showing the reactions between NBD-Cl and a set of thiols. HPLC peak area (Section 3.2.3) of NBD-SR products after 20 min of reaction in solutions containing NBD-Cl (2 M), CnHSH (2 M, red columns), and C6FSH (2 M, green columns) in the presence of (b) GMP andC10FTACN·Zn2+(50 M each) or (c) TMP andC16cyclen·Zn2+(30 M each). Concentration of NBD-SC4Hand NBD-SC6Fafter 20 min of reaction in solutions containing NBD-Cl (2 M), C4HSH (1.5 M), and C6FSH (4.5 M) in the presence of (d) GMP andC10FTACN·Zn2+(50 M each) or (e) TMP andC16cyclen·Zn2+(30 M each) (Section 3.2.4). Error bars indicate the standard deviation calculated from the duplicate experiments. Experimental conditions: Zn2+(600 M), [HEPES] = 5 mM, pH 7.0,T= 25 °C.
