Line Plot from Scientific Research

Stern–Volmer plot for fluorescence quenching of ATTO 655 covalently attached to microgels (μG) by surrounding H2O molecules. The ratio of the fluorescence lifetimes in pure D2O,τf,0, and the fluorescence lifetimeτfat the respective H2O/D2O solvent mixture is plotted against the (bulk) molar fraction of water,. The data for 22 °C (swollen state), 30 °C (just below the VPTT), 34 °C (just above the VPTT), and 40 °C (collapsed state) are presented in blue, cyan, orange, and red, respectively. The dashed lines are linear fits. For comparison, the Stern–Volmer plot for ATTO 655 in pure water at 22 °C is shown in black. The latter is very similar to the corresponding plot for 40 °C as shown in the Supporting Information.

DOI