FL-SMLM images and corresponding fluorescence lifetime distributions of microgels at 22 °C (left part of Figure) and 40 °C (right part of Figure) and for three different H2O/D2O-ratios (pure H2O in a, d, g, j; 50 % D2O and 50 % H2O in b, e, h, k; pure D2O in c, f, i, l), respectively. The FL-SMLM images are intensity-weighted lifetime distributions color-coded in the corresponding fluorescence lifetime. Each point cloud represents a microgel. The same color-code is used for the fluorescence lifetime distribution histograms. The average fluorescence lifetimes are also noted. Extended images and the results for additional H2O/D2O-ratios can be found in the Supporting Information.
