Catalytic enzyme genes are shown in italic. Blue italics represent genes with significantly different expression, and the adjacent bar graphs show the log 2 scale of the fold change (log 2 FC) of differentially expressed genes (DEGs) after knockout or reversal based on RNA-seq. The blue bars (Δ3 K) represent the comparison between C4599 and C4599Δ3K, the yellow bars (WT+hp1-4) represent the comparison between C1789::hp1-4 and C1789pEasy, and the red bars (WT+hp5) represent the comparison between C1789::hp5 and C1789pEasy. Wald test adjusted for multiple testing using the procedure of Benjamini and Hochberg was used to test significance, and adjusted p value < 0.05 was considered statistically significant when comparing the wildtype and its mutant. *adjusted p < 0.05, * *adjusted p < 0.01, ****adjusted p < 0.0001. Source data are provided as a Source Data file. Metabolic pathway reference: https://www.kegg.jp/entry/map00270 .
