The effect of depleting the genes obtained from the DSCC1-KD CRISPR–Cas9 screen alongside the cohesin genes WAPL and PDS5A. RPE-1 DSCC1 conditional KO cells (DSCC1 Δ/flox cre tam) were transfected with either scrambled (SCR) siRNAs or siRNAs against the indicated gene in the presence of 100 nM 4-OHT; viability was assessed in comparison to the parental cell line (SCR; 4-OHT). The experiment was repeated n = 3 independent times (biological replicates in technical triplicate). The timeline of siRNA and 4-OHT addition is indicated. Note that the y axis is displayed on a log 10 scale. Data are mean ± s.d. Statistical analysis was performed using two-tailed Student’s t-tests.
