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Derived cleaved amplified polymorphic sequence (dCAPS) markers were used to map crossovers within HOTSPOT6 ( HS6 ) and calculate cM/Mb. The numbers printed above the plot line show the number of crossovers identified in each interval. The midpoints of crossovers mapped by sequencing, as in Fig. 1 , are indicated as purple ticks along the top axis. Gene annotation (red) is shown underneath, in addition to Gypsy/Ty3 (blue), LINE1 (dark blue), MuDR (green), hAT (pink), and Helitron (brown) transposon annotations.

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