Immunostaining of sections of optic nerve from zebrafish with lymphatics labelled for Mrc1a (white arrows; a , top left) and iDISCO immunolabelling of meningeal lymphatic vessels of rabbit ( a , top right), pig ( a , bottom left), non-human primate ( a , bottom right) and human ( b ) optic nerve and chiasma, with lymphatics showing colocalization of LYVE1 and VEGFR3 (white arrows). DAPI, 4',6-diamidino-2-phenylindole. Scale bars, 50 m ( a , top left; c , bottom merged), 500 m ( a , top right; c , top merged), 1,000 m ( a , bottom left and right) and 3,000 m ( b ).
