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sCLNs, dCLNs or both CLNs were surgically ligated. Two days later, dye was injected into the eye through the AC ( f ) or IVT ( g ) route. The percentage of dye retention in the eye was measured 12 h later. Data are shown as mean s.e.m. in f and g (sham AC, n = 3; n = 4 in all other conditions). **** P < 0.0001; *** P = 0.0002, IVT sCLN; *** P = 0.0003, IVT dCLN; NS, not significant. P values were calculated using a one-way analysis of variance (ANOVA) with multiple comparisons testing (Dunnett).

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