Quantification of transgenic neurons. Each data point represents a sum of all HA-tag + cells counted from 36 fields of view of an independent well and normalized with DAPI and MAP2, shown as a percentage of the of 1-week condition. Between 281 and 2,680 cells were analysed per data point. n = 4 independent wells. Unpaired t-test for 1 week versus 2 weeks time points for each condition. Data shown are mean s.f. Two-tailed P values. EF1-driven NPTX2HA 2 week versus 1 week, P = 0.0009; TRE-driven TDP-43HA 2 week versus 1 week, P < 0.0001; EF1-driven TDP-43HA 2 week versus 1 week, P < 0.0001.
