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Schematic showing a functional B 12 acquisition system, including the periplasmic binding protein BtuF and ABC transporter BtuCD. The phylogenetic tree of BtuC was constructed based on the alignments of 762 protein sequences with 281 aligned positions. The branches are colored according to the taxonomic source of these sequences, and the assumed five clades, along with the outgroup, are labeled on the phylogenetic tree. Mmc, Methanomassiliicoccales; M. stadtmanae , Methanosphaera stadtmanae

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