Microscopy from Scientific Research

Mouse embryonic submandibular salivary gland (E14) was cultured ex vivo for 5 days, then labeled by bathing concurrently with Rhobo6 and 6-carboxyrhodamine 110. The latter dye differs from Rhobo6 only in that it does not contain the two ortho -aminomethylphenyl boronic acid groups, which are necessary for binding to extracellular glycans. Images were denoised (see Extended Data Figure 4b,c for comparison of raw and denoised salivary gland images; Supplementary Table 1 details the image-processing workflow for all datasets).

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