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RT-qPCR quantification of CycT , Cdk9 and DV-regulated genes ( dpp , zen , ind , sog , twi , and sna ) mRNA levels (relative to 28S rRNA) in wild-type embryos and P-TEFb maternally overexpressed (OE) embryos. Error bars show SEM. Significant differences in mRNA (two tailed, unpaired t -test) are indicated by asterisks (* = P < 0.05, ** = P < 0.01, *** = P < 0.001).

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