Growth and morphogenesis of mouse embryonic salivary glands upon incubation with Rhobo6; n = 14 glands were split into two paired groups, with each pair corresponding to glands from a single embryo. The first group was incubated with 5 M Rhobo6 in medium containing 0.5% DMSO, and the second group was incubated in medium containing 0.5% DMSO, as a vehicle control. Growth and morphogenesis were assessed by counting epithelial buds every 24 h for 2 days. Paired groups were compared using paired two-tailed t -tests; error bars represent s.d.; n.s., not significant.
