Scatter Plot from Scientific Research

Illustration for how deMULTIplex2 models tag cross-contamination in a simple two-sample multiplexing experiment. Each sample is labeled with tag A or B. Pooling the samples for single-cell capture allows floating tags to be bound to the cell or captured by the droplet. These contaminating tag counts are modeled by fitting two GLM-NB models in two separate spaces, and the sample identity is inferred using the EM algorithm. The y = x line is shown in black for this panel and panels C , D , F .

DOI