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Positive predictive values for all gRNAs across all time points. The predicted positives are defined as the top 3 predicted most efficient gRNAs for each gene, while the positive class includes gRNAs within N-fold of the depletion value of the most strongly depleted gRNA for each gene ( N = 1.5–5 with a step size of 0.5). The boundaries of the shaded regions and points indicate PPV values for each time point. The genes purE and purK were excluded in B – D .
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