FLIM microscopy of MCF10A+GFP-MUC1CT cells labeled with Rhobo6. Top, phasor plot of lifetime distribution, with ROIs marking unbound and bound Rhobo6 populations. Bottom, intensity contrast image compared with lifetime bandpass images for each population (Supplementary Table 1 ). The broad distribution of observed lifetimes could be due to a multitude of possible binding modes, as well as variability in glycan structure. g , cosine transform; s , sine transform.
