Confocal microscopy of MCF10A cells labeled with Rhobo6. Expression of GFP-MUC1CT was induced through the addition of doxycycline. Mucin domains, which are amino-terminal to GFP, were degraded enzymatically through live-cell treatment with the mucinase StcE 36 . Mucin overexpression in these cells induces membrane protrusions (villi), which are resolved as a thick halo around the cell; mucin overexpression also causes the cells to partially lift from their growth substrate, resulting in a spherical appearance with no loss in viability 35 . Contrast is normalized for each channel across experimental conditions.
