Response characteristics of the decoder circuit. For each condition, 60 g of agarose-immobilized split TEV, 30 g of agarose-immobilized split TVMV, and 40 g of agarose-immobilized Casp3 were immobilized on their respective magnets in 550 L assay buffer. The four conditions comprise: no input, novobiocin (53.6 m), EDTA (10 mm), or novobiocin (53.6 m) + EDTA (10 mm). Samples were taken from the supernatant for each condition, and all three protease activities were measured for each input. Data indicate the protease activities after 5 h, bar charts show the mean of two independent biological replicates for each condition.
