Workflow from Scientific Research

Open access visualization of Workflow, Illustration, Photometry Recordings, Optogenetic Stimulation, Bitter Neurons
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Schematic of photometry recordings from GCbt, and optogenetic stimulation of bitter neurons in the rostral NST (rNST). GCbt was targeted with AAV-CamKII-GCaMP6s, and the rNST of SST-ires-Cre mice was targeted ipsilaterally with an AAV-EF1alpha-DIO-hChR2 virus. A recording fiber for GCaMPs photometry was implanted above GCbt, and a stimulating fiber was positioned above the bitter neurons expressing ChR2 in the rNST.

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