Schematic of photometry recordings from GCbt, and optogenetic stimulation of bitter neurons in the rostral NST (rNST). GCbt was targeted with AAV-CamKII-GCaMP6s, and the rNST of SST-ires-Cre mice was targeted ipsilaterally with an AAV-EF1alpha-DIO-hChR2 virus. A recording fiber for GCaMPs photometry was implanted above GCbt, and a stimulating fiber was positioned above the bitter neurons expressing ChR2 in the rNST.
