NOX4 protein expression is upregulated in control but not in Piezo1-depleted HL-60-derived PMNs at 2 h post-transmigration through the Transwell system with 5-μm-diameter pore. NOX4 was not upregulated in fMLP-exposed PMNs grown in suspension or in non-transmigrated PMNs. Data are obtained from 3 independent experiments.