Piezo1 and downstream expression of NOX4 are required for ROS generation in transmigrated PMNs. ROS production measured in HL-60-derived PMNs stably transduced with control, Piezo1, or NOX4 shRNA lentiviral particles. ROS concentration was assessed at 1.5 h post-PMN transmigration through the microfluidic system. Data are obtained from 3 independent experiments. Additional information is provided as Figure S6.
