Illustration from Scientific Research

Schematic of the method used to separate TM and non-TM PMNs across lung microvessels. All PMNs fromLy6GCre td-Tomato +/- mice expressed td-Tomato as shown in Figure S1A. Intravascular PMNs were additionally labeled by alpha-Ly6G-BV421 antibody injected i.v. 2 min prior to euthanasia. The number of extravasated td-Tomato PMNs relative to intravascular PMNs stained with alpha-Ly6G-BV421 was calculated to quantify trans-vascular PMN migration.

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