ChIP-qPCR assay shows the CTCF, BRCA2, RAD51 binding, and H2A.X modification around the DSB sites. DD-Sce-I-GR DRGFP U2OS cells were treated with 0.5 M Shield1 and 0.2 mM TA for 0.5, 1, 2, 4, 6, 12, or 24 h before collection for ChIP-qPCR. Two genomic regions (-180 bp and +150 bp) adjacent of DSB sites were monitored. Cells were transfected with TET1 CD/dCD. The recruitments were quantified relative to the IgG control.
