CLEM workflow for tissue-scale vEM, applied to localize GFP-tagged T. gondii tachyzoite cells (green) inside the hindbrain of a zebrafish larva (macrophages, magenta; nuclei, blue).90A full vibratome section was imaged in 3D by confocal fluorescence microscopy (FM, top left) and by serial block-face SEM (SBF-SEM, bottom left). Colored boxes correspond to regions in different SBF-SEM slices containing single (cyan, middle) and replicating (orange, right) tachyzoites (marked with green arrows). Scale bars from left to right: 20 μm, 500 nm, 500 nm.
