Left: 3D segmentation of a C. reinhardtii cell generated by cryo-PFIB-SEM imaging, showing some of the cellular ultrastructure that can be resolved by this vEM technique, including thylakoid membranes (green) and the pyrenoid (dark yellow) in the chloroplast; mitochondria (light yellow); the nucleus (blue) containing the nucleolus (cyan); and numerous vacuoles, ER, and Golgi (shades of red and orange). Note the two cilia extending from the apical side of the alga (gray). Right: virtual slice through a cryo-ET volume on the edge of a C. reinhardtii chloroplast, showing the morphology of the thylakoid membrane network. Note the densities protruding from the thylakoid membranes, which correspond to photosynthetic membrane proteins. Scale bar: 50 nm.
