Gel from Scientific Research

5hmC Dot blot assay. Cells were treated with 150 M TMZ, 0.5 mM D-2-HG, 10 mM alphaKG, TET1 or TET2 overexpression for 24 h, 400, 200, 100, 50, 25, 12.5 ng gDNA was used for the assay. 5hmC was monitored with the antibodies.

DOI