Results for the mix containing all 25 strains (ALL mix) grown with a tetracycline (1st gen), b doxycycline (2nd gen), and c tigecycline (3rd gen). X-axis labels denote antibiotic-concentration-timepoint (hours). Left panel: barplots of the relative abundance of each strain in the starting mix, then after growth in the antibiotic selective conditions at 0.5x, 1x, and 4x the MIC of the empty vector strain. Bar height represents the average of three replicates. Middle panel: the percent change in the summed relative abundances of each strain belonging to a given mechanism relative to the starting mixture. Right panel: the percent change in the relative abundance of specific strains from a given mechanism. Points represent the average of three replicates, and error bars represent the standard deviation. TET tetracycline, DOX doxycycline, TIG tigecycline, EFF efflux pump, RPP ribosomal protection protein, DES1 type 1 tetracycline destructase, DES2 type 2 tetracycline destructase.
