Colony-forming units (CFUs) recovered from feces of animals (y axis) 2 days after inoculation with the indicated doses of EcN WT (x axes). A gray bar indicates the threshold of colonization required for protection against sorbitol-induced diarrhea. LOD, limit of detection.*p < 0.05;**p < 0.01;***p < 0.005;****p < 0.001.