Gel from Scientific Research

The CARD8 inflammasome is required for the rapid loss of CD4+T cells. Unstimulated CD4+T cells with indicated knockouts were mixed with autologous CD8+T cells at a 2:1 ratio before co-culture with HIVNL4-3-producing cells. Cas9 editing efficiency was confirmed in (D). The immunoblots represent four independent experiments. Four blood samples were used. p values were calculated using two-way ANOVA with Šidak’s multiple comparison tests.*p < 0.05,**p < 0.01, ns: not significant. Error bars show mean values with SEM from three or four independent blood donors. See alsoFigure S2.

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