AlphaFold 2 predicted structure of the catalytic domain of MobA with single amino acid variants from all 4,516 human gut metagenomes superimposed as ball-and-stick residues. oriT DNA (gray) and a Mn2+ion marking the active site (purple) were modeled based on 4lvi.pdb.82The size of the ball-and-stick spheres indicates the proportion of samples carrying variation in that position (the larger the sphere, the more prevalent the variation at the residue), and the color is in CPK format. The color of the ribbon diagram displays the values of the AlphaFold2-generated per-residue confidence metric, predicted local distance difference test (pLDDT), where the color red indicates very high confidence (>90 pLDDT) and the color orange indicates high confidence (>80 pLDDT). See alsoTables S3andS4.
