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Place-LED controlled stimulation of VTA VGlut2:ChR2 neurons biases behavioral preference. Top, experiment design. Mice explored the test enclosure equipped with two place-LED controllers (10-min sessions). The first (VTA light-Off) session allowed the identification of the initial preference of each mouse for one place-LED set. In subsequent light-On sessions, the place-selective activation of the initially non-preferred LED resulted in 4-Hz-patterned light pulse delivery to VTA VGlut2:ChR2 neurons. These sessions alternated with additional light Off sessions (laser inactivated). Bottom, example animal paths in VTA light-Off (left) and light-On (right) sessions. Colored lines show animal path segments in LED trigger zones.

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