Line Plot from Scientific Research

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Quantification of place-LED preference using a difference estimation plot. Top, raw data points, with each group of five connected points reporting the difference between the initially non-preferred (paired with VTA 4-Hz photo-stimulation) place-LED (Set 2) minus the preferred (unpaired) place-LED (Set 1) activation for one mouse across sessions; bar charts, average (mean SEM) preference. Lower panel, corresponding effect size, using the mean difference for each session compared to the initial light-Off session. Black dot, mean difference; filled curve, distribution of mean differences; black lines, 95% confidence interval;***p < 0.001;*p < 0.05; repeated ANOVA for pairwise session-session interactions versus initial light-Off session, with Bonferroni correction for multiple comparisons.
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