Left: schematic of the co-culture experiment between mouse melanoma YUMM1.7 cells expressing the ovalbumin (OVA) antigen and CD8+T cells isolated from OT-1 mice. Right: cell/membrane damage measured by lactate dehydrogenase (LDH) release in melanoma cell cultures exposed to a siRNA pool control (si CTRL) or a siRNA pool targetingTCF4(siTCF4) in the absence (CTRL) or presence of CD8+T cells. Individual values, mean, and SEM are shown (n = 3 technical replicates, Tukey’s multiple comparison test,∗adjusted p < 0.05,∗∗adjusted p < 0.01,∗∗∗∗adjusted p < 0.0001).
