Left: schematic of the co-culture experiment between melanoma A375 cells engineered to express the NY-ESO1 antigen (NYE-A02) and the immortalized T cell Jurkat cells engineered to express selectively the receptor against NY-ESO-1 (NYE-TCR). Right: percentages of CD69+cells in Jurkat T cells 24 h after exposure to autologous A375 tumor cells expressing the antigen NY-ESO-1 in whichTCF4was silenced using a siRNA pool (siTCF4). Cells exposed to a control siRNA pool (siCTRL) were used as control. Melanoma cells exposed to the same siRNA pools and cultured in absence of Jurkat cells were used as negative control (CTRL). Jurkat cells only were treated with phorbol myristate acetate (PMA) and ionomycin (Iono) and used as positive controls. Individual values, mean and SEM are shown (n = 3 technical replicates, Tukey’s multiple comparison test,∗∗∗∗adjusted p < 0.0001).
