Multiplex immunostaining (CODEX) and RNAscope images of a representative treatment-naive lymph node metastasis. CD45, CD31, and TCF4 (yellow, cyan, and white, respectively) protein stains are shown, whereas mRNA of the two selected genes for the MLGs (S100A1,CDH19,SOX10plusMITF; red) and MES state (DCN,TCF4,THY1, andLUM; green) are combined (top). A color-split of each four genes is shown (bottom). Nuclei of MES cells co-expressing MLGs and MES genes are segmented based on DAPI staining (dark blue). See alsoFigure S3.
