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In vivo real-time measurement of physiological parameters in seedlings expressing fluorescent sensors. Normalized average sensor ratios after autofluorescence correction for indicated physiological parameters are plotted, SE (n = 4–6). Chemicals were added at 0 h (after 1 h pre-incubation). Up/down arrows represent significantly higher/lower levels of the parameter. Asterisks represent the first time point, with statistically significant difference in treated versus mock (p < 0.05).
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