Change in upwind velocity and ground speed, calculated as the difference in the mean value during the first 5 s of odor and the 5 s preceding odor, across various control and experimental genotypes. Knockdown of Imp results in significant decreases in upwind velocity change when compared with respective controls, across multiple odor strengths and driver lines (pointed-GAL4 > mCherryRNAi compared with pointed > double ImpRNAi p = 5.906 × 10−11, pointed-GAL4 > mCherryRNAi compared with pointed-GAL4 > ImpRNAi(III) p = 1.14768 × 10−9, pointed-GAL4 > mCherryRNAi (1:10 AVC) compared with pointed-GAL4 > double ImpRNAi (10:1) p = 1.2293 × 10−4, DL1 > mCherry compared with DL1 > double ImpRNAi p = 9.922 × 10−6). Knockdown of late transcription factor Syncrip (Syp) results in a significant increase in upwind velocity when using a higher odor concentration (1:10 ACV, pointed-GAL4 > mCherryRNAi compared with pointed-GAL4 > SypRNAi [10:1] p = 0.0042). Knockdown of Imp using a single copy of the RNAi results in a significant decrease in ground speed (pointed-GAL4 > mCherryRNAi compared with pointed-GAL4 > ImpRNAi [III] p = 0.0015), as does knockdown in DL1/DL2 type II NSCs (17A12-GAL4 > ImpRNAi; ImpRNAi, two-sample t test; p = 2.2281 × 10−7). Upregulation of Imp results in increased ground speed but no change to upwind velocity (pointed-GAL4 > mCherryRNAi compared with pointed-GAL4 > UAS-Imp p = 7.7113 × 10−4). All comparisons were completed using an unpaired t test with Bonferroni correction for multiple comparisons. See alsoFigures 4,S1, andS2.
