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Schematics describing the genetics of the CLIn system combined with a heat shock promoter for genetic birth dating: type-II-specific stg promoter fragment combined with KD recombinase removes a STOP sequence by a flip event, and an intact Flippase is reconstituted. This, combined with a heat-sensitive promoter, removes the STOP sequence and leaves Cre recombinase active in type II NSCs in a heat-shock-dependent manner. Cre, in turn, generates type II NSC clones positive for LexA::p65. GAL80 is active in the rest of the cells, and GAL4 expresses GFP as a reporter in a class-specific manner, whereas mCherry downstream of LexAop sequence is expressed as a reporter in a lineage-specific manner.

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