Workflow from Scientific Research

Genetic scheme for type II lineage analysis using type-II-specific flip-out. Ase-GAL80 active in type I NSCs leaves Wor-GAL4 active only in type II NSCs. Flippase downstream of a UAS sequence is activated in a type-II-specific manner and removes the STOP sequence between two FRT sites. This causes expression of reporter GFP in class-specific manner when crossed to a class-specific LexA driver line.

DOI