CD45+cells from the tumor microenvironment (TME) of 4T1P(205,678 cells) and 4T1M(236,251 cells) tumors were segregated into 25 distinct, unsupervised clusters. A heatmap of normalized, scaled cluster frequencies per-sample is shown. Cluster genotypes and parental cell-types were annotated based on the expression of all markers, inspected in parallel (see Figure S1B). Generalized linear models (GLMs) were fit to detect differentially abundant (4T1Pvs. 4T1M, combined treatments) clusters. Treatment was initiated at a tumor size of ∼50 mm3(arrow). Significance was assessed by means of FDR-corrected, Bayesian-moderated t tests (*, FDR < 0.01;**, FDR < 0.001;***, FDR < 0.0001).
