Flow cytometric analyses of myCAFs (CD45-CD31-EpCAM-PDPN+Ly6C-) and iCAFs (CD45-CD31-EpCAM-PDPN+Ly6C+) from the PDPN+ gate in tumors derived from the transplantation of PDAC organoids with or without EgfrWT or EgfrKO PSCs. Results from 4 separate experiments (n = 4–5 mice/cohort/experiment). PDAC+EgfrWT PSC tumors and PDAC+EgfrKO PSC tumors were compared to the PDAC alone tumors (T) of each individual experiment. This is needed to evaluate the effect of Egfr deletion on CAF composition across co-transplantation experiments performed with different PDAC organoid and PSC lines that generate tumors with various degrees of CAF composition and abundance.
