Significantly downregulated pathways (i.e., NES < −1.50 and FDR < 0.25) identified by gene set enrichment analysis (GSEA) ofEgfrKO PSCs (n = 8) compared toEgfrWT PSCs (n = 4), both co-cultured with PDAC organoids for 4 days in Matrigel in control media. (C and H) Thein vitroandin vivoCAF signatures were obtained from Öhlund et al.9and Elyada et al.,6respectively. Results shown as mean ± SD (A and B) or mean ± SEM (D and E),∗, p < 0.05;∗∗, p < 0.01;∗∗∗, p < 0.001, paired Student’sttest. See alsoFigure S3andTables S2,S3.
