enzyme-linked immunosorbent assay (ELISA) of AREG in murine parental (i.e., unmodified), WT (i.e.,Rosa26KO),Tgfbr2KO,EgfrKO andErbb2KO PSCs cultured for 4 days in Matrigel in control media with or without 20 ng/mL TGF-β in the presence or absence of 1 μM erlotinib (EGFRi) or 300 nM neratinib (ERBBi) or 2 μM A83-01 (TGFBR1i). Results show mean ± SEM of n = 4–14 (F) or n = 4–13 (G) biological replicates.
