Gel from Scientific Research
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RT-PCR shows that the transferred Arabidopsis mRNAs were associated with B. cinerea polysomes. The transferred plant mRNAs were shifted from the polysome fractions to the monosome fractions upon puromycin treatment. Ten fractions of equal volume were collected from top to bottom of 15% to 55% sucrose gradients. Treatment of puromycin or not is as indicated (+ or ).
#Gel#RT-PCR#Arabidopsis mRNAs#B. Cinerea Polysomes#Plant mRNAs#Polysome Fractions#Monosome Fractions#Puromycin Treatment#Sucrose Gradients
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