Only the transferred wild-type SAG21 and APS1 mRNAs but not the mutated versions were translated into tagged proteins in fungal cells. Samples from (D) were subjected to immunoblot using -GFP as primary antibody (right panels). Size markers are indicated in KD, and protein loading is represented by Ponceau staining (PS). See also Figures S3 and S4, and Table S4.