Gel from Scientific Research

Phos-tag SDS-PAGE gels assessing phosphorylation status of fluorescently labeled (*) BC Ubc8 (top) and UBE2H (bottom) after their incubation with ATP/MgCl2-supplemented yeast and HEK293 lysates, respectively, with or without inhibitors of CK1, CK2 and GSK3. C-terminally truncated versions of BC Ubc8 (157-218, CTE) and UBE2H (162-182, CTE) lacking phosphorylation sites were included as negative controls.

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